Coding

Part:BBa_K5048065:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-23)


SUMO-HSP60-FLAG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 215
    Illegal PstI site found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 215
    Illegal PstI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 215
    Illegal BglII site found at 104
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 215
    Illegal PstI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 215
    Illegal PstI site found at 4
    Illegal NgoMIV site found at 619
    Illegal AgeI site found at 774
    Illegal AgeI site found at 1252
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Note

SUMO: Ubiquitin-like protein SMT3. A protein tag with a molecular weight of 11.3 kDa, is used to enhance protein solubility.
We build up this gene circuit to obtain more protein HSP60 in the supernatant, aiming to acquire more raw material for Co-IP(Fig 1).

Fig 1 The genetic circuits for SUMO-HSP60.


Source

SMT3 SUMO family protein SMT3 [ Saccharomyces cerevisiae S288C ] Gene ID: 852122


References